The Nanoscale World

Single Particle Tracking

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posted on Thu, Nov 3 2011 10:35 AM

Single Particle Tracking can be used to track proteins (or different molecules) in a fluid membrane to measure their diffusion coefficient. But as the particle has an intrinsic mass, the measured value is articifical and the real value significantly underestimated.

I would like to know if any of view has some abaques/tables on this. Usually the particles are made of the same material but I would like to be able to put some metrics on this, like:

for a 20 nm particle, the diffusion coefficient will be decreased by factor 2, 3... or something.

Thanks in advance,

Alex.

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Top 75 Contributor
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Hi Alex,

sorry I don't have answer on your question.

Could you tell how did you implement (which technique) single particle tracking and on which instrument?

Thanks,

Dejan

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replied on Tue, Dec 13 2011 10:54 AM

Hi Dejan,

 

Sorry for the misunderstanding: I never used that technique so far so I have no idea what is required in terms of setup; I was just curious how the values mentioned in the literature are relevant;

Thanks anyway,

Alex.

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Top 75 Contributor
15 Posts
Points 178

Ok Alex.

Could you tell me in which literature did you find these values?

Best,

Dejan

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replied on Wed, Dec 14 2011 3:13 AM

Not really; this was a while ago during my PhD. I was working on FRAP at that time and was interested in how you can measure the diffusion coefficient of proteins, and remember quite a number of publications where people used SPT. But I still believe that this speed of diffusion is strongly affected by the mass of the particle. Sorry not to be able to provide you better information.

Alex.

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Top 75 Contributor
15 Posts
Points 178

Alex,

thank you anyway!

Best,

Dejan

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