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I would like to learn how to image hydrophilic active surfaces, e.g. Nylon,
and wonder if anybody actually did that. I have tried to image in air but apparently the surface pull off the probe.
Therefore I did those measurements in HEPES, which apparently seems to give a better contrast image.
However I am not entirely sure about the quality of those images.
In principle, there should be no problem imaging Nylon in air (it should be simpler than in fluid).
I have seen many hydrophilic and hydrophobic polymers in air.
What actually happened when you tried to image it in air?
One tip: Try drying the sample thoroughly, hydrophilic
samples can end up with a lot of absorbed water in air,
and this can cause some imaging problems.