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I posted the topic a few months ago. Just to inform you that the publication is eventually available in Journal of Phycology. Here is the abstract:
QUANTITATIVE NANOMECHANICAL MAPPING OF MARINE DIATOM IN SEAWATER USING PEAK FORCE TAPPING ATOMIC FORCE MICROSCOPY Galja Pletikapic, Alexandre Berquand, Tea Misic Radic and Vesna Svetlicic It is generally accepted that a diatom cell wall is characterized by a siliceous skeleton covered by an organic envelope essentially composed of polysaccharides and proteins. Understanding of how the organic component is associated with the silica structure provides an important insight into the biomineralization process and patterning on the cellular level. Using a novel atomic force microscopy (AFM) imaging technique (Peak Force Tapping), we characterized nanomechanical properties (elasticity and deformation) of a weakly silicified marine diatom Cylindrotheca closterium (Ehrenb.) Reimann et J. C. Lewin (strain CCNA1). The nanomechanical properties were measured over the entire cell surface in seawater at a resolution that was not achieved previously. The fibulae were the stiffest (200 MPa) and the least deformable (only 1 nm). Girdle band region appeared as a series of parallel stripes characterized by two sets of values of Young’s modulus and deformation: one for silica stripes (43.7 Mpa, 3.7 nm) and the other between the stripes (21.3 MPa, 13.4 nm). The valve region was complex with average values of Young’s modulus (29.8 MPa) and deformation (10.2 nm) with high standard deviations. After acid treatment, we identified 15 nm sized silica spheres in the valve region connecting raphe with the girdle bands. The silica spheres were neither fused together nor forming a nanopattern. A cell wall model is proposed with individual silica nanoparticles incorporated in an organic matrix. Such organization of girdle band and valve regions enables the high flexibility needed for movement and adaptation to different environments while maintaining the integrity of the cell.
QUANTITATIVE NANOMECHANICAL MAPPING OF MARINE DIATOM IN SEAWATER
USING PEAK FORCE TAPPING ATOMIC FORCE MICROSCOPY
Galja Pletikapic, Alexandre Berquand, Tea Misic Radic and Vesna Svetlicic It is generally accepted that a diatom cell wall is characterized by a siliceous skeleton covered by an organic envelope essentially composed of polysaccharides and proteins. Understanding of how the organic component is associated with the silica structure provides an important insight into the biomineralization process and patterning on the cellular level. Using a novel atomic force microscopy (AFM) imaging technique (Peak Force Tapping), we characterized nanomechanical properties (elasticity and deformation) of a weakly silicified marine diatom Cylindrotheca closterium (Ehrenb.) Reimann et J. C. Lewin (strain CCNA1). The nanomechanical properties were measured over the entire cell surface in seawater at a resolution that was not achieved previously. The fibulae were the stiffest (200 MPa) and the least deformable (only 1 nm). Girdle band region appeared as a series of parallel stripes characterized by two sets of values of Young’s modulus and deformation: one for silica stripes (43.7 Mpa, 3.7 nm) and the other between the stripes (21.3 MPa, 13.4 nm). The valve region was complex with average values of Young’s modulus (29.8 MPa) and deformation (10.2 nm) with high standard deviations. After acid treatment, we identified 15 nm sized silica spheres in the valve region connecting raphe with the girdle bands. The silica spheres were neither fused together nor forming a nanopattern. A cell wall model is proposed with individual silica nanoparticles incorporated in an organic matrix. Such organization of girdle band and valve regions enables the high flexibility needed for movement and adaptation to different environments while maintaining the integrity of the cell.
Galja Pletikapic, Alexandre Berquand, Tea Misic Radic and Vesna Svetlicic
It is generally accepted that a diatom cell wall is characterized by a siliceous skeleton covered by an organic envelope essentially composed of polysaccharides and proteins. Understanding of how the organic component is associated with the silica structure provides an important insight into the biomineralization process and patterning on the cellular level. Using a novel atomic force microscopy (AFM) imaging technique (Peak Force Tapping), we characterized nanomechanical properties (elasticity and deformation) of a weakly silicified marine diatom Cylindrotheca closterium (Ehrenb.) Reimann et J. C. Lewin (strain CCNA1). The nanomechanical properties were measured over the entire cell surface in seawater at a resolution that was not achieved previously. The fibulae were the stiffest (200 MPa) and the least deformable (only 1 nm). Girdle band region appeared as a series of parallel stripes characterized by two sets of values of Young’s modulus and deformation: one for silica stripes (43.7 Mpa, 3.7 nm) and the other between the stripes (21.3 MPa, 13.4 nm). The valve region was complex with average values of Young’s modulus (29.8 MPa) and deformation (10.2 nm) with high standard deviations. After acid treatment, we identified 15 nm sized silica spheres in the valve region connecting raphe with the girdle bands. The silica spheres were neither fused together nor forming a nanopattern. A cell wall model is proposed with individual silica nanoparticles incorporated in an organic matrix. Such organization of girdle band and valve regions enables the high flexibility needed for movement and adaptation to different environments while maintaining the integrity of the cell.
It is generally accepted that a diatom cell wall is
characterized by a siliceous skeleton covered by an
organic envelope essentially composed of polysaccharides
and proteins. Understanding of how the organic
component is associated with the silica structure provides
an important insight into the biomineralization
process and patterning on the cellular level. Using a
novel atomic force microscopy (AFM) imaging technique
(Peak Force Tapping), we characterized nanomechanical
properties (elasticity and deformation) of
a weakly silicified marine diatom
Cylindrotheca closterium
(Ehrenb.) Reimann et J. C. Lewin (strain CCNA1).
The nanomechanical properties were measured over
the entire cell surface in seawater at a resolution that
was not achieved previously. The fibulae were the
stiffest (200 MPa) and the least deformable (only
1 nm). Girdle band region appeared as a series of
parallel stripes characterized by two sets of values of
Young’s modulus and deformation: one for silica
stripes (43.7 Mpa, 3.7 nm) and the other between the
stripes (21.3 MPa, 13.4 nm). The valve region was
complex with average values of Young’s modulus
(29.8 MPa) and deformation (10.2 nm) with high
standard deviations. After acid treatment, we identified
15 nm sized silica spheres in the valve region
connecting raphe with the girdle bands. The silica
spheres were neither fused together nor forming a
nanopattern. A cell wall model is proposed with individual
silica nanoparticles incorporated in an organic
matrix. Such organization of girdle band and valve
regions enables the high flexibility needed for movement
and adaptation to different environments while
maintaining the integrity of the cell.