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Polysaccharide - solution

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paul posted on Tue, Feb 7 2012 7:20 AM

Anybody having experience in imaging polysaccharides in liquid?

Paul

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Biopolymers. 1996 Mar;38(3):355-66.

Imaging polysaccharides by atomic force microscopy.

Source

Institute of Food Research, Norwich Laboratory, UK.

Abstract

Techniques have been developed for the routine reliable imaging of polysaccharides by atomic force microscopy (AFM). The polysaccharides are deposited from aqueous solution onto the surface of freshly cleaved mica, air dried, and then imaged under alcohols. The rationale behind the development of the methodology is described and data is presented for the bacterial polysaccharides xanthan, acetan, and the plant polysaccharides l-carrageenan and pectin. Studies on uncoated polysaccharides have demonstrated the improved resolution achievable when compared to more traditional metal-coated samples or replicas. For acetan the present methodology has permitted imaging of the helical structure. Finally, in addition to data obtained on individual polysaccharides, AFM images have also been obtained of the network structures formed by kappa-carrageenan and gellan gum.

Found this in the literature. I would love to read the article but unfortunately I don't have journal access.

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