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请教化学方法修饰针尖

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Answered (Verified) This post has 2 verified answers | 5 Replies | 2 Followers

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H Yang posted on Thu, Mar 7 2013 1:37 AM

孙博、各位大侠，请教一下如果是用化学方法修饰针尖，想得到带羧酸根或者氨基基团的，应该选用哪几种化合物呢？反应的过程中有哪些注意事项？不胜感激！

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Answered (Verified) Hao Sun replied on Sun, Mar 10 2013 8:04 PM
Verified by Hao Sun

可以用镀金的探针，一端是巯基-SH，它可以和Au形成共价键，另一端是与被测物有特异相互作用的基团，比如要检测羧基，可以用氨基等；中间可以用PEG调节链的长短。留下邮箱，我发给您一些资料。

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Answered (Verified) Hao Sun replied on Mon, Mar 11 2013 8:16 PM
Verified by H Yang

已经发送了两篇关于针尖修饰的App notes，请注意查收。

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Answered (Verified) Hao Sun replied on Sun, Mar 10 2013 8:04 PM
Verified by Hao Sun

可以用镀金的探针，一端是巯基-SH，它可以和Au形成共价键，另一端是与被测物有特异相互作用的基团，比如要检测羧基，可以用氨基等；中间可以用PEG调节链的长短。留下邮箱，我发给您一些资料。

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H Yang replied on Mon, Mar 11 2013 1:11 AM

非常感谢哈孙博，最近刚开始修饰针尖遇到的问题比较多。我的邮箱地址：yanghui@iccas.ac.cn。

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Answered (Verified) Hao Sun replied on Mon, Mar 11 2013 8:16 PM
Verified by H Yang

已经发送了两篇关于针尖修饰的App notes，请注意查收。

  • | Post Points: 12
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H Yang replied on Wed, Mar 20 2013 2:05 AM

孙博，文献已经收到了，感谢哈~

文献中用的是Olympus biolever的AFM探针，这种就是镀金的探针吗？就是说把Si或者SiN3的针尖镀上金的吗？在化学修饰过程中有哪些注意事项呢（比如清洗针尖之类的细节），还有就是如何判断已经修饰成功了呢？再次感谢！

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Hao Sun replied on Fri, Mar 22 2013 1:16 PM

以前有篇文献，判断修饰与否的方法是将待修饰的物质连接荧光基团，若修饰成功可以观察到探针上的荧光。

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