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Streptavidin-coating of surfaces

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Not Answered This post has 0 verified answers | 2 Replies | 1 Follower

Top 200 Contributor
5 Posts
Points 52
Brent Nelson posted on Fri, Feb 24 2012 5:50 PM

Hi all -

I've been trying to track down a protocol for functionalizing glass or mica surfaces with streptavidin.  Does anyone have a good protocol or know of a good online resource?

 

thanks,

Brent Nelson

Assistant Professor of Mechanical Engineering

Northern Arizona University

  • | Post Points: 12

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Top 10 Contributor
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Ang Li replied on Sun, Feb 26 2012 8:00 PM

Hi, Brent,

Since mica is negative charged and PI of strepavidin is ~5, so if you have strepavidin in an acidic solution, you should be able to direct deposit strepavidin on mica surface through charge interaction.

LA

  • | Post Points: 12
Top 200 Contributor
5 Posts
Points 52
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Brent Nelson replied on Tue, Feb 28 2012 2:17 PM

Thanks - do you have any literature references by any chance?

 

Brent

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