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Why in the Adhesion calculation only minimum force of adhesion well is taken into account, not work of adhesion (the integrated pull-off area)? It might introduce substantial error, and even an opposite contrast in adhesion map.
The Adhesion channel measures the adhesion force which is dominated by the Capillary force in the air. The work of Adhesion is desired but not included in this version of software. But user can use High Speed Data Capture to capture up to 32 second deflection error signal and use Nanoscope Analysis program to export the corresponding force curves and calculate the work of adhesion offline.