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Single Particle Tracking can be used to track proteins (or different molecules) in a fluid membrane to measure their diffusion coefficient. But as the particle has an intrinsic mass, the measured value is articifical and the real value significantly underestimated.
I would like to know if any of view has some abaques/tables on this. Usually the particles are made of the same material but I would like to be able to put some metrics on this, like:
for a 20 nm particle, the diffusion coefficient will be decreased by factor 2, 3... or something.
Thanks in advance,
Alex.
Hi Alex,
sorry I don't have answer on your question.
Could you tell how did you implement (which technique) single particle tracking and on which instrument?
Thanks,
Dejan
Hi Dejan,
Sorry for the misunderstanding: I never used that technique so far so I have no idea what is required in terms of setup; I was just curious how the values mentioned in the literature are relevant;
Thanks anyway,
Ok Alex.
Could you tell me in which literature did you find these values?
Best,
Not really; this was a while ago during my PhD. I was working on FRAP at that time and was interested in how you can measure the diffusion coefficient of proteins, and remember quite a number of publications where people used SPT. But I still believe that this speed of diffusion is strongly affected by the mass of the particle. Sorry not to be able to provide you better information.
Alex,
thank you anyway!