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I recently attended the Bruker workshop in Manchester, UK and had the opportunity to meet Michael Sherratt from the University of Manchester.
His team recently published a paper in Matrix Biology (29 (2010) 254-260 about AFM imaging of fixed human skin tissues and I was totally amazed by the quality of the pictures they acquired on their Multimode (N III controler). As far as I know, the best existing collagen images. Here is the abstract if you want to know more about this:
Tissue section AFM:
In situ ultrastructural imaging of native biomolecules
Conventional approaches for ultrastructural high-resolution imaging of biological specimens induce
profound changes in bio-molecular structures. By combining tissue cryo-sectioning with non-destructive
atomic force microscopy (AFM) imaging we have developed a methodology that may be applied by the nonspecialist
to both preserve and visualize bio-molecular structures (in particular extracellular matrix
assemblies)
in situ. This tissue section AFM technique is capable of: i) resolving nm–μm scale features of intra- and extracellular structures in tissue cryo-sections; ii) imaging the same tissue region before and after experimental interventions; iii) combining ultrastructural imaging with complimentary microscopical and micromechanical methods. Here, we employ this technique to: i) visualize the macro-molecular structures of unstained and un
intra- and extracellular structures in tissue cryo-sections; ii) imaging the same tissue region before and after
experimental interventions; iii) combining ultrastructural imaging with complimentary microscopical and
micromechanical methods. Here, we employ this technique to: i) visualize the macro-molecular structures of
unstained and un
fixed fibrillar collagens (in skin, cartilage and intervertebral disc), elastic fibres (in aorta and lung), desmosomes (in nasal epithelium) and mitochondria (in heart); ii) quantify the ultrastructural effects of sequential collagenase digestion on a single elastic
and lung), desmosomes (in nasal epithelium) and mitochondria (in heart); ii) quantify the ultrastructural
effects of sequential collagenase digestion on a single elastic
fibre; iii) correlate optical (auto fluorescent) with ultrastructural (AFM) images of aortic elastic lamellae.
with ultrastructural (AFM) images of aortic elastic lamellae.