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In soft tapping you start with a small free amplitude (Afree) and you use a relatively large setpoint to free amplitude ratio (Asp/Afree as large as possible, but usually greater than about 90%). Note that Afree should be measured within a few um of the sample since the cantilever is damped during the...
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Q: Is there any output in the QNM mode which is comparable to the phase imaging in tapping? What is the difference between Inphase and Quadrature outputs, in layman terms? Why does the quadrarture seem to have more noise? A: The inphase is the Rcos(theta) and quadrature is the Rsin(theta) outputs from...
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See this new Nano Letters paper on ultrahigh resolution imaging and mechanical mapping of bacteriorhodopsin. http://pubs.acs.org/doi/abs/10.1021/nl202351t. I thought I would post this link as nice example of the science that can be done using the AFMs high resolution imaging power, coupled with the inherent...
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PS: A good test of the robustness of a system is to scan Celgard® in different orientations; here is an even faster video (23Hz) where the mesh is perpendicular to the scan. Enjoy, Steve
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I was reading an AFM site and saw a contrived claim trying to make a comparison that was >50% off the benchmark and thought it would be a good topic to open up for discussion. The comment was to the effect of: by scanning at a slower scan speed we are actually going faster because our poor scanner...
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It’s good to see some community attempts at replicating the performance of the Dimension FastScan. The screen shot below is of a 1um, 512x512, 22Hz, Video of Celgard® taken with the Dimension FastScan (Video link at end). This demonstration is over twice as fast as the 10Hz video we show above...
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Nothing like a quality sample preparation (thanks Natalia!) and an awesome AFM to get great soft-sample high-speed images (thanks Shuiqing & Adam!). This new video shows height and phase of freshly prepared Celgard; the images are at ~20um/s (or 10Hz scan rate for 1um images): In the video, we also...
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Interesting debate going on linked-in. Reposting here for comment as well. **** Normal 0 false false false EN-US X-NONE X-NONE What qualifies a as a high speed AFM? There has been a lot of discussion on what it takes to claim high speed. Should a baseline criteria be to demonstrate at least an order...
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For those interested in dynamic imaging with the AFM: Attached is a link to a Dimesnion FastScan video of DNA taken in tapping mode in fluid at a rate 1 frame per second. We took this data in collaboration with Y. Lyubchenko, at the University of Nebraska Medical Center, as a proof of concept experiment...
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Today Bruker Nano Surfaces Division announced the innovative and unique Dimension FastScan AFM, which delivers a significant breakthrough in improved imaging speed without sacrificing nanoscale resolution. The Dimension FastScan enables users to obtain usable data significantly faster than is possible...