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In soft tapping you start with a small free amplitude (Afree) and you use a relatively large setpoint to free amplitude ratio (Asp/Afree as large as possible, but usually greater than about 90%). Note that Afree should be measured within a few um of the sample since the cantilever is damped during the approach by air confined between the cantilever
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Q: Is there any output in the QNM mode which is comparable to the phase imaging in tapping? What is the difference between Inphase and Quadrature outputs, in layman terms? Why does the quadrarture seem to have more noise? A: The inphase is the Rcos(theta) and quadrature is the Rsin(theta) outputs from the lockin that is driving the PFT modulation (usually
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See this new Nano Letters paper on ultrahigh resolution imaging and mechanical mapping of bacteriorhodopsin. http://pubs.acs.org/doi/abs/10.1021/nl202351t. I thought I would post this link as nice example of the science that can be done using the AFMs high resolution imaging power, coupled with the inherent information available from mechanical tip
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PS: A good test of the robustness of a system is to scan Celgard® in different orientations; here is an even faster video (23Hz) where the mesh is perpendicular to the scan. Enjoy, Steve
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I was reading an AFM site and saw a contrived claim trying to make a comparison that was >50% off the benchmark and thought it would be a good topic to open up for discussion. The comment was to the effect of: by scanning at a slower scan speed we are actually going faster because our poor scanner dynamics require so much rounding we have to make
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It’s good to see some community attempts at replicating the performance of the Dimension FastScan. The screen shot below is of a 1um, 512x512, 22Hz, Video of Celgard® taken with the Dimension FastScan (Video link at end). This demonstration is over twice as fast as the 10Hz video we show above. As you start to see different images and movies
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Nothing like a quality sample preparation (thanks Natalia!) and an awesome AFM to get great soft-sample high-speed images (thanks Shuiqing & Adam!). This new video shows height and phase of freshly prepared Celgard; the images are at ~20um/s (or 10Hz scan rate for 1um images): In the video, we also tried to showcase a lot of the ease-of-use features
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Interesting debate going on linked-in. Reposting here for comment as well. **** Normal 0 false false false EN-US X-NONE X-NONE What qualifies a as a high speed AFM? There has been a lot of discussion on what it takes to claim high speed. Should a baseline criteria be to demonstrate at least an order of magnitude improvement over a conventional AFM on
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For those interested in dynamic imaging with the AFM: Attached is a link to a Dimesnion FastScan video of DNA taken in tapping mode in fluid at a rate 1 frame per second. We took this data in collaboration with Y. Lyubchenko, at the University of Nebraska Medical Center, as a proof of concept experiment. Here we use the enhanced bandwidth of the system
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Today Bruker Nano Surfaces Division announced the innovative and unique Dimension FastScan AFM, which delivers a significant breakthrough in improved imaging speed without sacrificing nanoscale resolution. The Dimension FastScan enables users to obtain usable data significantly faster than is possible with other commercial AFM systems. It is simply