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See this new paper on ultrahigh resolution imaging and mechanical mapping of bacteriorhodopsin. http://pubs.acs.org/doi/abs/10.1021/nl202351t. I thought I would post this link as nice example of the science that can be done using the AFMs high resolution imaging power, coupled with the inherent information available from mechanical tip-sample interaction
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I have received a number of questions if FastScan (our High Speed AFM) works with ScanAsyst (our Auto-Optimization algorithms). It does - and this video link demonstrates that by showing unattended high speed imaging on a diverse set of challenging samples. (http://www.youtube.com/user/BrukerNano?feature=mhee#p/u/0/7bi2YEgie_k) In the video are several
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When compared to other common microscopy techniques (optical, SEM, TEM), the atomic force microscope’s (AFM’s) broad potential for nanoscale imaging and characterization of numerous physical surface properties has been somewhat offset by its slow imaging speed. Thus, the AFM has sometimes been seen as a powerful “specialty tool”
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It’s good to see some community attempts at replicating the performance of the Dimension FastScan. The video is of a 1um, 512x512, 23Hz, scan of Celgard® taken with the Dimension FastScan. This demonstration is over twice as fast as the 10Hz video we showed previously, additionally we demonstrate the Celgard® with the mesh oriented a couple
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HF Etched Mica. The video shows a Survey at 20um @ 4.8Hz, followed by a high resolution image at 4um @ 20Hz, followed by a video at 0.66um 55Hz. Hard flat samples are not that challenging for a high speed system because they don't challenge the z-actuator or slew rate (power) of the electronics.
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I was reading an AFM site and saw a contrived claim trying to make a comparison that was over 50% off the benchmark and thought it would be a good topic to open up for discussion. The comment was to the effect of: by scanning at a slower scan speed we are actually going faster because our poor scanner dynamics require so much rounding we have to make
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It’s good to see some community attempts at replicating the performance of the Dimension FastScan. The screen shot below is of a 1um, 512x512, 22Hz, Video of Celgard® taken with the Dimension FastScan (Video link at end). This demonstration is over twice as fast as the 10Hz video we show above. As you start to see different images and movies
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Nothing like a quality sample preparation (thanks Natalia!) and an awesome AFM to get great soft-sample high-speed images (thanks Shuiqing & Adam!). This new video shows height and phase of freshly prepared Celgard; the images are at ~20um/s or 10Hz scan rate for 1um images. In the video, we also tried to showcase a lot of the ease-of-use features
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Bruker Dimension Fastscan video of a 180nm Grating at 11Hz, 30um
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DNA on Mica in Fluid (Prep by APS Method) Sample courtesy of Y. Lyubchenko, Univ. of Nebraska Med. Ctr 1 Second per image (2100 Frames were captured at 1 frame per second)