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Usually the tips which are really damaging to the cells are the ones: 1) that have a silicone (not Si3N4 but pure silicone) ending. 2) that have a pyramidal shape (like the OTR for instance, which are very good to image proteins but not suitable for cells). Let me know how it goes. Good luck, Alex.
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Simon Scheuring U1006 INSERM / AIX-MARSEILLE UNIVERSITE Tel. ++33 (0)4 91 82 87 08 (Secretary) Tel. ++33 (0)4 91 82 87 77 (Office) Email simon.scheuring@inserm.fr www: http://u1006-inserm.univ-mrs.fr We are seeking a highly motivated and talented postdoc for developing and applying a combined atomic force microscopy (AFM) – tip enhanced Raman
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Hi Eike-Christian, I suggest opening a new workspace and check if the problem presists. Regards, Stefan
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Hi yark, What are the version numbers of your software packages (aquisition and analysis)? Did you save the files any different than your other files? Did the problem occur again or did just happen once? Thanks, Stefan
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Hi Peter, Did you contact: http://www.asmicro.com/ It might be worthwhile checking with Don if he has what you are looking for. Best, Stefan
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Thanks very much for the very prompt answer. I'll tell my customer. Best, Alex.
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Wow... 100nm; that's a lot! For 5-10 Gpa, the TAP525 are really the best. The RTESPA are slightly too soft and the DNISP slightly too stiff. Best, Alex.
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I got the following question from a user and was not able to answer: Does anybody know if Si3N4 and Si probes stand Piranha treatment? Thanks, Alex.
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I think my answer didn't go through... Once again: I hardly use Hertz on cells but in that case, the equation is fairly easy. If using Sneddon, the correct anglt should be the BACK angle (25 deg. in that case). May I ask your opinion about those probes (I never had a chance to test them) in terms of measured modulus and quality/stability of imaging
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Dear Jeff, I spend most of my time working on biological samples with QNM but I also have experience with QNM on much stiffer samples (up to 100 GPa). I noticed that whatever the sample, the tips often get contaminated and/or dulled after even just one scan. Of course this severely impacts on the measured Young's modulus. If you start from a tip