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Could someone please recommend to me the best (and simplest) way of doing the following: (1) Comparing two images (that have drift between them) to find the best possible overlap (I'm guessing a correlation program of some sort). (2) Then trimming both images so they show the same area. (3) Subtracting the resulting images from one another. I know
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Can someone describe, or better still, give a reference to a standard method for determining noise levels in AFMs? In particular, I'm interested in a method applicable to compare noise levels across different microscopes, so it should not be dependent on a particular scope's software.
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The RMS roughness has an statistical value that the roughness average or surface area data don't have. The RMS roughness is nothing but the standard deviation of the height values contained in your AFM image. So it is a well-known statistical quantity that is used everywhere. Ideally, the RMS value tells you that around a 60% of the points of the
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Can anyone clearly explain to me the value of RMS? Why would I want to give someone Root Mean Square data vs. Roughness Average or surface area data? Any help on this would be most appreciated.
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Folks using the Surface Force Apparatus to measure intermolecular and/or surface forces have been using thermosetting resins to adhere their silvered mica sheets to the crossed cylinders for years with no adverse effects. Epon resins 1004 and 1009 are suitable for aqueous solutions up to 60C.
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Does anyone have a script handy that can convert Omicron Scala image files to the DI Nanoscope format?
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For those who are interested in cleaning and contamination control of AFM probes including colloidal probes, I would like to invite you to read our recent Ultramicroscopy article entitled "Cleaning AFM Colloidal Probes by Mechanically Scrubbing with Supersharp "Brushes", 2009, vol.109, issue 8, 1061-1065". Please click this link
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In principle, there should be no problem imaging Nylon in air (it should be simpler than in fluid). I have seen many hydrophilic and hydrophobic polymers in air. What actually happened when you tried to image it in air? One tip: Try drying the sample thoroughly, hydrophilic samples can end up with a lot of absorbed water in air, and this can cause some
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I would like to learn how to image hydrophilic active surfaces, e.g. Nylon, and wonder if anybody actually did that. I have tried to image in air but apparently the surface pull off the probe. Therefore I did those measurements in HEPES, which apparently seems to give a better contrast image. However I am not entirely sure about the quality of those
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We had low SUM on one of our Dimension 3100's and ended up sending the scanner head to Veeco for repair (can't remember if they swapped the laser, the detector, or something in between). They provided a loaner scanner so we could keep working in the meantime. Repair was not too expensive.