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  • Re: PeakForce Tapping with OBL

    Janne, with respect to the engage gain, usually 0.3 (up to 0.4) should be enough. But that's usually for levers of 0.1 N/m (or higher). For very soft levers like OBLs, I would say 0.2. Regarding your 2nd question, what I would try is: 1) Engage in contact mode or force volume mode. 2) Go to Ramp and update the deflection sensitivity. 3) Withdraw
    Posted to SPM Digest (Forum) by Anonymous on Wed, Nov 14 2012
  • Re: dynamic spring constant

    Chris, Though I am not a physicist, my guess is that it's static in air and dynamic in fluid but I am not 100% sure. I found this link you might find interesting: http://silver.neep.wisc.edu/~msallen/Frentrup-MSThesis-AFMCalibrationNonunif.pdf Chapters 2, 3 and 4 of this thesis are mentioning that. Best, Alex.
    Posted to SPM Digest (Forum) by Anonymous on Fri, Nov 9 2012
  • Re: PeakForce Tapping with OBL

    Hi Jane, Modify the engage setpoint was a good idea. You can also try to increase the engage gain. If this is not enough, you can go into the Microscope---Engage Settings and decrease the SPM engage step (which is 15 um by default). Also (I guess you already know that) make sure you wait at least 30 min before engaging. OBL probes are among the softest
    Posted to SPM Digest (Forum) by Anonymous on Fri, Nov 9 2012
  • AFM conference annoucement

    A nice conference happening in Okinawa end of this year: http://dora.bk.tsukuba.ac.jp/event/ICSPM20/introduction.en originally physics-oriented but they extended it to other areas like biology. Registration migh close very soon. Alex.
    Posted to SPM Digest (Forum) by Anonymous on Fri, Nov 9 2012
  • Re: How to use Point and Shoot Method to Calculate Modulus in Live Cells using the Catalyst

    Hi Iman, Yes, HSDC is another way to do it. With respect to your weird numbers, there are 2 possibiities: 1) Your defl sens / k / tip radius were not properly calibrated. 2) For the vast majority of the curves, you were too close to the substrate. Please check your average indentation vs the cell thickness. If you are too close, no wonder you find very
    Posted to SPM Digest (Forum) by Anonymous on Fri, Oct 19 2012
  • Re: How to use Point and Shoot Method to Calculate Modulus in Live Cells using the Catalyst

    Hello, Question 1. This is fairly easy: once you hav processed the curve, select Run History, select a destination folder and drag & drop all the curves into the main pannel. Then just press Run. But if you have 5000 force curves, it might take you a while... (several minutes). Question 2. You have several options but the easiest is probably to
    Posted to SPM Digest (Forum) by Anonymous on Thu, Oct 18 2012
  • Re: Height differences between tapping and Peak force tapping measurements. Any reference?

    If your height issmaller in tapping it is an indication at you indent your soft bio sample more than you do in PeakForce tapping. One has to carefully analyze the forces involved to understand that. When I am back in the office I will check on the publication. Stefan
    Posted to SPM Digest (Forum) by Anonymous on Thu, Sep 27 2012
  • Re: drifting in successive height scans

    It for sure will be the tip and sample that gets heated. The diffraction limited spot from the IR laser is so big that it will cover both tip and sample. I checked but unfortunately to this point could not find any software routine that would help you with the drift correction. Stefan
    Posted to SPM Digest (Forum) by Anonymous on Thu, Sep 27 2012
  • Re: How can we do EFM measurements on BioScope Catalyst? What hardware is required?

    Some EM measurements are possible with the Catalyst but not SICM. As I am not a specialist of electrical modes, I hope somebody will follow-up on that post. Regarding your question about P&S, it's quite easy to do this on a horizontal line: just select the "line" option and "2" in the "number of points" tab. Then
    Posted to SPM Digest (Forum) by Anonymous on Mon, Sep 24 2012
  • Re: drifting in successive height scans

    Hi Eric, The info on the NanoDrive file format is already on here. What do you do to minimize drift? Is your room temperature stable? Stefan
    Posted to SPM Digest (Forum) by Anonymous on Mon, Sep 17 2012
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