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  • Re: Multimode AFM and cell

    SNL probes are not specific to any one mode of operation. They are widely used for contact mode, tapping mode and force measurements. Because they are especially sharp, SNL probes may not be the best choice for live cell imaging. Our DNP probes are better suited for that since they are somewhat less sharp and therefore less likely to puncture, tear
    Posted to SPM Digest (Forum) by Ben Ohler on Fri, Aug 26 2011
  • Re: Stepper motor problem

    It sounds as though either the motor or the motor drive electronics may have been damaged. Please check with our Service group. Contact details by region can be found here . Regards, -Ben
    Posted to SPM Digest (Forum) by Ben Ohler on Fri, Aug 26 2011
  • Re: STM Head Converter for Multimode System

    Hi, I'm the product manager for the MultiMode. If you send me a note with your contact details I will be happy to put you in touch with your local sales person. Regards, -Ben ben.ohler(at)bruker-nano.com
    Posted to SPM Digest (Forum) by Ben Ohler on Thu, Aug 25 2011
  • Re: FSUB-11 substrate for bio samples

    As I recall, the substrate are a bit smaller, about 10x10mm square such that they fit conveniently in the MultiMode. The substrates are amine group functionalized. As such, they are single use only. The idea is to use a glutaraldehyde linker to bind proteins to the amine surface. Some further details are available in this Application Note . Please check
    Posted to SPM Digest (Forum) by Ben Ohler on Thu, Aug 25 2011
  • Re: How can I filter force curves?

    Hi, there's a couple useful tools within NanoScope software to do this. They are described in more detail in the "NanoScope Software Tools for Force Spectroscopy" application note found here . Briefly though, "Filter Curves" works by defining a minimum force that must occur at some minimum distance from the contact point. This
    Posted to SPM Digest (Forum) by Ben Ohler on Wed, Aug 3 2011
  • Re: PF QNM Tip qualification

    The titanium roughness sample for tip characterization (model RS-15M) is available from the Bruker AFM Probes website ( link ). This version is premounted on a 15mm sample disk. It's also available unmounted as model RS. Once you image the sample you can then analyze it using our free NanoScope Analysis software ( link ) to estimate the tip shape
    Posted to SPM Digest (Forum) by Ben Ohler on Tue, Aug 2 2011
  • Re: Dependence of deflection-sensitivity on ramp-size

    Hi Jonas, To calibrate deflection sensitivity calibration reliably here are a few suggestions: 1) Always calibrate on a clean sample that won't be indented significantly by your probe. The NSC36A probe has a nominal spring constant of 0.95 N/m. It should be fine to calibrate this on glass or silicon. For stiffer probes you might need to use a harder
    Posted to SPM Digest (Forum) by Ben Ohler on Mon, Jul 25 2011
  • Re: TUNA module on DI3100 with Nanoscope IIIA

    Hi Shaomin, Sorry, it's not possible to use application modules like TUNA on older systems with the original Extender module. They require at least the Quadrex Extender, which is a separate box like the original Extender that can be used with NS3a and NS3D controllers. Quadrex was also built into NSIV controllers. Application modules are also supported
    Posted to SPM Digest (Forum) by Ben Ohler on Fri, Apr 15 2011
  • Re: lateral deflection of cantilever on flat surface

    It's hard to see your data clearly, but it doesn't look like your lateral deflection data is random from what I can see. I think you will find that it can be understood in terms of how the cantilever experiences a torque when pressed against surfaces with different slopes. This issue has been looked at quite a bit as it relates to calibrating
    Posted to Off-Topic (Forum) by Ben Ohler on Tue, Mar 22 2011
  • Re: BioHeater accessory for AFM

    Sorry, there is not a cooling stage available for the Innova. Its only temperature control option is an ambient to 60°C heater. Both heating and cooling (-35°C to 250°C) are available on our MultiMode, Dimension 3100 and Dimension Icon product lines. Regards, -Ben
    Posted to SPM Digest (Forum) by Ben Ohler on Fri, Mar 18 2011
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