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Hi Joey, To add to Adam's note, be aware that you can use the thermal tune function in the NanoScope software together with the Sader method calculators linked by Adam to implement the Sader method. You just follow the thermal tune process halfway, collecting the spectrum and then fitting the peak to the simple harmonic oscillator model. This yields
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Hi Dalia, I'll add a bit to what Steve has already written. My RSI paper that he linked is a good starting point. All of the AFM data in it was taken on a MultiMode. As you note, it really comes down to signal to noise ratio. As that term isn't well defined in this context, I presented my own definition in the paper. I looked at the ratio of
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Hi Anjani, Bruker's offline force curve analysis tools are described in this Application Note: NanoScope Software Tools for Force Spectroscopy Data Analysis . In particular, the "Multiple Curve Analysis" function is very effective at automatically finding and tabulating the maximum adhesion in each retraction curve in a large batch of
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Hi Ash, Unfortunately the thermal tune function doesn't have separate parameters for the "prefactor" (i.e. 0.971 or 0.965) and the X (Chi) squared deflection sensitivity correction. There is only a single correction factor that is treated like Chi (i.e. squared in the denominator). In other words, the equation it uses is like Eqn 16 in
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Guillaume, Those are two recent conference presentations, so I'm not sure how much detail will appear in the journal itself. Our most detailed explanation to date is the PeakForce QNM application note . Regards, -Ben
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Guillaume, The correction should be set to 1.106 for rectangular cantilevers and 1.144 for v-shaped cantilevers. This is left as a numeric parameter rather than just a shape selection since some users may prefer other corrections. A full discussion of the correction can be found in my spring constant calibration application note . An explanation of
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Hi Andreas, I did use the box-counting method to analyze some lipid monolayer domain images for my dissertation. We only looked at a couple images and we didn't consider it a very critical analysis, so we didn't put any work into automating the analysis or otherwise making it easy. I forget the exact details, but we literally just drew different
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Guillaume, Often the lateral resolution of force volume is simply limited by the lower pixel resolution that is typically used. Typically we use 64x64 or 128x128 grids instead of say 512x512 or higher for normal imaging. In this case your best resolution would simply be the scan size divided by the number of pixels. It's just not practical to use
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You will find the Hertz model used in almost all papers that analyze AFM nanoindentation. It exists in two common forms, one modeling the indentation of a flat surface by a sphere (what I present above) and the other modeling the indentation of a flat surface by a cone (often referred to a Sneddon model). Manfred Radmacher at University of Bremen is
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Hi Guillaume, What range of deflection sensitivities are you measuring in fluid? They will vary somewhat from the values measured in air, but not by a lot. The most common issue calibrating cantilevers in fluid is that the resonant frequency shifts to much lower frequencies and the peak becomes much wider. For soft cantilevers, the peak will extend