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Hi Beauzamy, Hopefully the following suggestions might make exporting your data a little easier. To extract your force curves as text files, you will need to save the baseline corrected curves. Once you have saved all these curves (either by applying the baseline correction individually or using the Run History function) then select all the corrected
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Can you verify if you are using the relative trigger mode? Using the absolute trigger will likely give you different max applied force values depending on your deflection at the start of each force curve. Also, which probe are you using? I have not tried to apply such high forces much on Bio-samples but I wonder if it might be possible that your probe
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Hi Sarah, You can install the NanoScope Software on another computer (other than your AFM system). During installation, select the Nanoscope installation type as 'Offline Workstation'. Once installed you can email Bruker to get a key to enable MIRO in this offline version of the software. This will allow you to open .iro files and manipulate
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Hi Solong, I am not so familar with conductive measurements but from the height image you definitely have a double tip. This can often happen in contact mode. It can be hard to tell if this is due to some defect of the probe or a result of the tip picking up some of the material from your sample surface. If the image started out looking good and then
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You should select the 'ramp and capture' option. Just 'ramp' only signals the AFM to perform the force measurements - it does not save the resulting force curves. With 'ramp and capture' the force curves will be saved in a folder with a file extension 'mca' (multi-curve analysis). If you open this folder in Nanoscope
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Hi Darice, The Sneddon model should be the better option for the modulus range of your samples. Unlike the DMT model, Sneddon does not use the tip radius so changing that shouldn't affect the modulus value in the Sneddon channel (you don't even need to calculate it). It does, however, use the tip half angle. You will need to set that in order
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Hi Benoit, Yes, you can use this probe for force volume measurements. Just be aware of how soft your sample is - as for samples like cells, bacteria and membranes this probe may be on the stiffer side (~0.7N/m). If you need a softer probe I've used the DNP-A probe (0.4N/m) which is about the same tip radius. Best, Andrea
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Hi Benoit, Which probes are you using? If you are using our ScanAsyst or DNP / MLCT probes then we use a nominal tip half angle of 18 degrees. Best, Andrea
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Hi Benoit, The deflection sensitivity of a probe does change depending on the particular AFM system you are using (different photodetectors) and depending on whether you are in fluid or air. If you are using a Bruker AFM with NanoScope software you should have access to a probes database that will give you a nominal deflection sensitivity for a particular
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Hello Philippe, When Fast Retrace is enabled in FV mode, the system does not perform force curves during retrace only during the trace. This is why your time to collect the FV image decreases by half. With respect to PeakForce Tapping, it is my understanding that the system does not 'record' each force curve because of the amount of time and