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Hi, I have seen this happen if you are ramping in open loop. Can you verify if you are ramping in closed loop (Z closed loop = ON) and monitoring Deflection/Force vs. Z sensor? Best, Andrea
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Hi Raphi, It's okay if you see a peak at 6-7 kHz rather than 8 kHz. I've actually found the position can vary between ~6-12 kHz depending on the probe and the probe holder itself. Thanks for including the screenshot of the frequency sweep in your post. That definitely helps. I'll assume that this is an off-surface sweep (tip withdrawn).
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Hi Alex, Not sure is this is what you're looking for but if you go to the American Type Culture Collection (ATCC) website ( www.atcc.org ) you can search for a particular cell line and it will provide you with the protocol for subculturing it, includuing whether or not you have to trypsinize and or/ centrifuge them. Hope this helps you! Best, Andrea
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Hi Raphi, You are using the same AFM probe (SNL-C) that we use for the majority of TappingMode in fluid applications. For fluid imaging we typically target a free amplitude of 300-500 nm depending on your sample. For this probe, 800 nm is not that uncommon of a drive amplitude to use to get these amount of free amplitude. The important thing is that
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Hi Luis, If you send me your email address we can get you a pdf copy of the On-Line Help. Best, Andrea
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Hi Sam, UV-ozone or plasma cleaning is more powerful for removing contaminants than just a UV lamp cleaner. Also, while I don't have any experience with metal-coated spheres, it is often recommended that you clean colloidal probes using either of these methods before using them. This helps to remove existing contaminants from the sphere that may
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Hi Shashikanth~ In some cases, high pass filtering does allow you to better observe features on the surface of cells in your height image. It is also comparable to looking at the error signal image (deflection, amplitude). This is why you will often see deflection or amplitude images shown in AFM cell studies - you do see 'edges' of the cells
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Hi Morgan~ Yes, the most common method of immobilizing DNA on mica is to use divalent cations, like NiCl2 and MgCl2, to passivate the negative charge on the mica and thus allow adsorption of the negatively charged DNA. Other groups use what we call "AP-mica" which is mica that has been silanized with an amino-terminated silane to give the
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A detailed procedure for the preparation of silanized mica (AP-mica) as a substrate for DNA imaging. Immobilization of DNA on the AP-mica is also described
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A detailed procedure for immobilizing DNA on a mica substrate using NiCl2-containing buffer solutions.