Greetings: I am using the AFM (Veeco NS 5, multimode scope) to indent soft biological tissues. I intend to use a soft cantilever (nominal stiffness = 0.01 N/m) with a 10 micron diameter glass or polystyrene particle attached to it (Novascan, Inc). I am looking for force-displacement curves to get the tissue stiffness. I would like to know how deep into