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Hi Stefan, Thanks for your reply. I found out the reason. I clicked the zoom toolbutton on scanning window before I opened the Probe Positioning Window. So the frame button became the zoom toolbutton instead of 2D array positioning button. Zhuoyang
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Hi, I am doing force curve measurement on Innova. When I use probe toolbutton to add locations, I found that a 2D array of locations cannot be added by dragging a frame on the obtained images. Only a frame appear and no locations can be selected. But a line of locations or individual locations can be added. I wonder if there is any restriction on this
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Hi Stefan, I couldn't find the application note #133 in the Bruker Media-Application notes. Could you please send me one copy? My email address is luzhuoyang@gmail.com. Thank you very much! Zhuoyang
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Hi, I often meet the problem that when large features occure on a flat surface, the substrate becomes artificially lowered and the images looks like shadows behind the features. How could I use the Nanoscope software to elimintate these shadows. Thanks. Zhuoyang
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Hi Stefan, Thanks for your answer. Our Nanodrive controller has an "IO-MOD+" board. Zhuoyang
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Hi, I want to reduce the imaging forces to about 50-100pN. How could I realise this in both contact and tapping mode? In contact mode, is it appropriate to adjust the setpoint until certain value that the forces are in the ranges above? In tapping mode, how could I adjust the drive amplitude to ~1nm, by reducing targeting tapping amplitude or setpoint
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Hi Stefan， I am applying AFM to organelle membrane patches. I haven't succeeded in scanning membrane proteins. Successfully engaging SNL probe is just a thing of a few days ago. At present only small protrusions of several hundreds of nm can be distinguished. When zoomed-in to a scan size of less than 100nm on the bilayer membrane, the profile began
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Hi Stefan, Could you please tell me whether the FM is better than AM, especially when scanning biological samples with ultra-high resolution in liquid. Is there any difference of "sensitivity" ? I read two papers scanning the same kind of sample, and it seems the FM image is clearer than AM image. Thank you! Zhuoyang
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Hi, I read about Frequency Modulation AFM in some papers. How can I realise the function of FM-AFM? Is it possible to do this by Open Hardware? Plus, the scanning mode is tapping in liquid, not EFM. Many thanks, Zhuoyang
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Hi Stefan, I think I have found out the reason. it indeed is far away from the sample surface. I set the distance of "withdraw tip" button to 0.4um, then engage (not successful), withdraw tip, and re-tune. I think the SNL resonace frequency is very strongly dependant on the distance between sample and tip.Now I can have the probe engaged successfully