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Hi, I am looking for any advice or recommendations for sample preparation in the case of in situ imaging of protein (antibody) adsorption on mica in fluid using tapping mode. I am using Nanoscope IIIa and MTFML liquid cell. I have problems with right sample injection procedure into the fluid cell, because I have constantly problems with fluid leaking
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Hi Dmitry, sorry for late reply. Yes, Skype is great idea. I will contact to to end of the week or next week (name: arzensekd)? Cheers, Dejan
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Hi, thank you for all suggestions. Stefan, I agree with you about using simple imaging procedure. But, at the beginning I want to estimate the range of setpoint amplitude which I could use and then is of course easier to use simplified procedure with known parameters. Ang, unfortionally I don't have access to scanasyst mode yet. And I agree that
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Hi Alex, yes, me again I am dealing with this really intensively these days and it seems that you are only one who is willing to reply on my questions . Now I am using approach which is written really extensively for imaging of antibodies in air by Neil H. Thompson and Santos (Cantilever dynamics in amplitude modulation AFM: continuous and discontinuous
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I am doing in tapping, so this should not has the great impact. I will try to estimate the possible tip contamination on uncoated tips. Maybe is the best way to characterize tip and then measure the adsorbed protein in fluid with the same tip to estimate possible contamination. And then hope that the contamination is insignificant. In the case of no
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No, I've never tried this, but sounds interesting. The only concern I have is that I am wondering if this could have influence on sample because of different tip-sample interactions (unfolding of protein due to hydrophobic tip contact?). Could you provide more information/details about this? Thanks for this suggestion, Dejan
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Thanks Alex Yes, I also think that you have right. I was asking that, because I saw in some literature (imaging of DNA from Lyubchenko) this approach. Do you have any advice with cantilever and fluid cell assembling to reduce any possibility of tip contamination? Now, I deposit the droplet of sample on freshly cleaved mica and assemble the fluid cell
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Hi, does the drying of the already adsorbed biological sample (like proteins) before immersing in liquid make any difference from adsorbed sample in liquid without intermediate drying step? With term "dried sample" I mean that it was prepared with procedure for imaging in air. I am asking this because I am concerned with contamination of tip
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Hi, I am performing high-resolution imaging of biomolecules (antibodies) in fluid using tapping mode. I am using Nanoscope IIIa and MTFML liquid cell. I know that amplitude depends on the distance of the tip from the surface and that there exist two stable regions (attractive at higher separations and repulsive at lower separations). In the literature
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Alex, thank you anyway! Best, Dejan