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Two types of PEG you may try out. One is Methyl-PEG-X (one end with CH3 and the other end with any active functional group such as NHS or malemide or biotin etc depending on what chemical group on your tip). The other is PLL-g-PEG, we used this as glass surface coating and it works nicely (but it's noncovalent bonding so might degrade faster for
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Hydrophobic tip may not be a good choice then since it will introduce more complex tip-sample interaction and current algorism may not be able to deal with it. We used to coat substrate surface with patterns of PEG coatings and it's very efficient to prevent cells attaching and force them to grow only in the PEG free regions. I believe PEG is a
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Hi, Alex, I could think of modifying your working tips to be hydrophobic (no need to worry about tip sharpness loss for cell imaging anyway), and a thin layer of PEG coating might worth a try, though I am not sure how it would affect the interaction between the tip and cell during scanning, your results will tell us I guess. LA
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Hi, Alex, We have some collaborators having some, you may contact me offline. LA
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Hi, Liz, I was thinking of your sample as imaging lipid like molecules so suggested in a liquid environment. Is your surface hydrophilic or hydrophobic and how is your molecules deposited, nonspecifically? and what would happen during drying process? Do you have other means (like optical imaging) to check your surface first to see the spreading of your
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Hi, Albert, FV channel in nanoscope software does not directly seperate into different property channels as Nanowizard II does. Instead, it shows the raw deflection data (thus in nm unit) at particular fixed z positions for each pixel. To extract stiffness and adhesion maps out from FV channel, you will need to choose proper z positions within indentation
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Hi, I would think of starting with scanasyst in fluid using normal SCANASYST-FLUID tips. Prob you will need more efforts on sample preparation then imaging part should be straightforward. Please update your progress here if anything unexpected come out so we can help you more specifically. LA
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Hi, Sam, In principle, you can have an AFM tip fixed on the substrate and scan over it using your microsphere tip, the image you get will reflect the surface of the microsphere and you just follow standard procedure to measure the roughness. The merit of this method is you can use different tips of varied sharpness to test the roughness you are interested
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@Ashkan You can find Alex's email in this post: http://nanoscaleworld.bruker-axs.com/nanoscaleworld/forums/t/563.aspx Can also send me a copy to: ang.li@bruker-nano.com LA
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Hi, Stefan, Prob I considered catalyst as special AFM for bio applications but existing users may not have it :) Nevertheless, Z range is one of the necessary specifications out of others like tip height and geometry and scanning mode for this application, and for living cells exceeding 4-5um high, I often find difficulty to get good images since the