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Hi Guys, Thank you for your useful responses. I have a problem opening my saved Hsdc. files. Every time I try to open a file, it opens up but when I click " QNM Hsdc Force Curve-Image" to open the file as it shown in help section, the software shows me this message: 'The QNMOffline needs either QNMOffline or HarmoniX or PeakForceQNM Key'
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Hi Alex, Thank you for your response. Could you please tell me what is the difference between DMT modulus and Young's modulus?! The Nanoscope analysis gives us the cell stiffness (from DMT channel) in DMT modulus whenever we measure the cell stiffness. However, when we do the HSDC we can extract the Youn'g modulus from the obtained curves. Am
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Hi Alex, Thank you for your useful advice. Could you please check if the procedure we are using is correct for opening a representative curve? We open the .hsdc file and select the deflection error channel (not the height channel) and load the associated image. From this, we select the curve we want and right click and select export XZ data. We then
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Hi all, In relation to this question, what would be the advantages of a .hsdc line file captured over the nucleus of a cell in addition to the area surrounding the nucleus versus the point and shoot method (where I would draw a box over the nucleus and take 20 indentation points for example). Which method would be the most accurate in determining the