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Just in case somebody else desperately tries to open the first link from above - you can find it here now: http://nanoscaleworld.bruker-axs.com/nanoscaleworld/media/p/143.aspx
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That's it. So easy. Thanks.
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Thanks nevertheless.
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Dear all, Is there a "close all"-function in NanoScope Analsysis, allowing to close all opened images at once? What I always do is to close the whole program and to start it once again as I don't want to close every single opened image individually. Regards, Dietmar
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Dear hyamanieu, Glad to read you managed to solve your problem. However, could you explain the solution you found once again, just in case somebody else has the same problem and finds this discussion looking for the answer? Regards, Dietmar
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Hello, Win Pin, It's a bit hard to judge like that what could be your problem, but could it be that your AFM does an Engage process first? Somehow the machine needs to know how far away it is from the surface and it gets this information while engaging. So it might not be possible for you to touch your cells only once I guess. However, you can play
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Hi, Sedeleo, They recommend to use "ScanAsyst" probes for a stiffness like that, which has a spring constant of 0.5 N/m. So your probe should be fine. Is the PDMS sample you mention a reference sample? If not, try to image the PDMS reference sample next time and try to create the very same force-distance curves from. They should look like
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Dear Sedeleo, Do you measure silicon or silicone? Your modulus sounds to me as to be too low for silicon. Your curves look awful. When I get curves like those, I readjust the laser point or even change the tip. Did you obtain better curves on a standard calibration sample, like saphire or the polystyrene calibration sample? The curves should be constant
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Still an open question ...
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Write me your email address, then I'll send you the manual I've got.