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Hi Sara, I believe I just switched to a new tip. It's unfortunate that PDMS is so sticky (sometimes we incubate in a small percentage BSA solution to reduce this), but it means that the tips must be switched out more often. The post above may also help, but I haven't seen it until now: indeed I was using trigger off mode when perhaps I should
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Hi Dietmar, Thank you for your quick reply. I misspoke, the measurements were taken on 1:10 PDMS. I was able to get pretty clean curves on polystyrene, as you mentioned. I can try to adjust the laser point next time. Would it suggest that the 0.95N/m tip is not appropriate for this sample? Thank you!
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We were able to zoom in and fit the curve more closely but spring constant still measures a high spring constant. It seems we will need a reference probe in order to correctly calibrate this probe.
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We are interested in measuring the rigidity of silicon surfaces that should range between 1 -3 MPa. The tip we are using is a 0.95N/m cantilever with a 4.5um polystyrene spherical tip. We previously used a 0.35N/m cantilever also with a 4.5um PS tip, but that was deemed too soft. In any case, the indentation of the material produced unusual curves.
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This is the first image, calibration in fluid And the second, calibration in air Thank you!
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Hello, Thanks for reading, hopefully some of you have encountered this sort of thing before. Our current probes are 0.95N/m rectangular cantilevers with 4.5um spherical tips - and we haven't been able to calibrate them successfully. Because these cantilevers are silicon probes, they are not coated - so our working voltage is only about 2.5-3.5