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Ash, There are many good references on calibration, here is one from Ben Ohler: AN094-RevA0-Practical_Advice_on_the_Determination_of_Cantilever_Spring_Constants-AppNote.pdf In general, the thermal tune calibration method takes into consideration the tip location because the method uses the measured deflection...
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The key is to get the calibration parms for the measurement conditions. If the coating will not effect the spring constant or tip end radius, you can do the calibration of those parms prior to coating. You cannot pre calibrate the deflection sensitivity since that depends on the laser alignment. In fact...
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Just learned about this product from Photonic Cleaning Technology today ( http://www.photoniccleaning.com/ ) Natalia cleaned this very dirty grating in about 15min. Optical images below: Before: After:
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The NSIVa thermal tune function is limited in frequency by the data acquisition rate of the NSIVa. The maximum frequency that can be tuned is about 30KHz. The data acquisition rate on the NSV is 50MHz, so it can tune cantilevers up to 2MHz with no problem (assuming that they are not too stiff). The thermal...
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I'm trying to demonstrate the tip width of a "1-nm radius" tip by scanning isolated features (bumps or ridges). Does anyone have a favorite specimen that has features 1 nm wide?
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How do you know the Modulus value for the reference sample?
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What standards ecan be used for PeakForce calibration? What range of moduli can be investigated?