In addition to its high resolution imaging capabilities, atomic force microscopy (AFM) has emerged as a powerful tool for measuring both the nanomechanical properties and interaction forces of biomolecular complexes. While the majority of these types of AFM studies have been conducted on isolated molecules, for true biological relevance these investigations should ideally be conducted on live cell systems. In addition, it now appears essential to be able to couple AFM with other techniques like optical microscopy in order to simultaneously collect both types of data. So far, very few studies are based on the combination of AFM and inverted optical microscopy.