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Dear all, I am trying to revive an old NSIIIa. Head, base and controller are working fine. The computer is running Windows XP. When I start the Nanoscope Software (V530r3rs3) I do not have the option for live-mode. Somehow the DSP card is not recognized by the software. In the system.par file IRQ is set to 11. The same is set in the BIOS of the mainboard
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Dear all, thanks for the support! After a few emails with James, the problem is more or less solved. If I want to approach the surface in tapping mode without crashing I have to choose a higher frequency. Because I am using an old liquid cell I first have to heavily increase the driving amplitude (~1800mV) to see peaks of about 150mV around 15-17kHz
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[quote user="Dmitry Sokolov"] Hi Raphi, please contact me for Web meeting: http://confocal-manawatu.pbworks.com/w/page/23665663/Dmitry%20Sokolov Cheers, Dmitry MIAWiki for Real-Time Collaboration [/quote] Hi Dmitry, thank you very much for your offer. I will contact you as soon as I have time! Cheers, Raphi
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Hey Ang, I use SNL-C at 6.9-7.2kHz, 500-800mV drive amp resulting in 200-300mV amplitude. Raphi
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Hi Alex, my default tapping engage parameters looks quite the same: - Delta Setpoint = -0.0600 -Final Delta Setpoint = 0.0600 -Test threshold slope = 25.0 -Test treshold dZ = 25.0 nm -Engage min setpt [%] = 25 -TM engage gain = 1.00 -Sew tip = Yes (here the only options I have are YES and NO. There is no TRIGGERED option) -Sewing trigger [%] = 98 (this
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Dear AFM specialists, I am using a Multimode V/Nanoscope 7.3 with tapping mode in liquid using a standard liquid cell with SNL probes. In the software, there are a bunch of engage parameters for tapping engage (Tools>Enage Settings>Tapping...; Page 91 in Nanoscope 7.3 Rev G User Guide). My problem is that I do not find the right parameters which
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Hi, Thank you all for your responses! I am really not sure if everything is working correctly in my setup. I just do not see any peak between 8 and 12kHz. It works with a frequency around 6-7kHz, maybe I should stick to this peak. Attached you will find a frequency sweep with SNL-C cantilever close to the surface <1um. There is no additional peak
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Hi Andrea, thx for your answer. The resonance frequency is always around 6.9-7.1 kHz. I never got a peak around 8... Do you use a special set of tapping engage parameters? Best, Raphi
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Hi, I try to improve our MM V AFM tapping operation in liquid. We normally use SNL-C (0.24N/m) cantilvers with a normal liquid cell (MTFML) in buffer solution (e.g. PBS). In literature I always find the lever/driving amplitude ratio is >1 for the same setup. In my case the ratio lies around 0.5-0.2 (e.g. 800mV driving amplitude results in 200-300mV
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Hey Dejan, In my case, I do not use any cross-linker at the moment. Freshly cleaved mica works fine... but maybe you can use AP-mica (APTES) or even glutaraldehyde.