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we published a paper using the two techniques, but seperately. I feel it unnecessary to run the two simultaneously.
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Hi, Djuenne, SNL may not be a good start point for imaging live cells, it's a bit too sharp and stiff. You may try MLCT first, its type C cantilever (the softest one with spring constant of 0.01N/m) is classical for imaging live cells in contact mode. The setpoint depends on your system and may vary dynamically due to thermally induced deflection
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Hi, Fenny, You can find some useful tips from a similar discussion: http://nanoscaleworld.bruker-axs.com/nanoscaleworld/forums/t/829.aspx The method I used often is to rinse the tip in ethanol followed by di water followed by plasma or UV ozone treat, you can also try stronger sovent as suggested in the above discussion if you still have the problem
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Hi, Fenny, Let me try to answer some of your questions: 1. On Catalyst, you have motorized stage, that means you can record xyz positions and move to particular positions accurately. So under Navigate, you will find the function to move automatically to absolute or relative xy position or you can just simply monitor the xyz values at the right bottom
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Hi, Linda, Before go into hardware diagnosis, have you tried different contact setpoint, especially trying to engage at higher force? Sometimes false engage would give you the same problem as you described, simply increase setpoint or allign laser at lower vertical deflection value may resolve the problem. Besides, are you using Multimode or Dimension
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sorry, witt, I didn't notice you are using MM, PFM is presently not a standard application on MM, the manual and protocol I mentioned are only for Dimension. So in such case, you may need to contact your local Bruker support for a customerized solution or upgrade to run your experiment. Prob Steve can help you if you need contact. LA
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Hi, I guess you should start with imaging in air instead of in liquid. The note is also for in air but not for in liquid. There should be little difference between different software versions. LA
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Hi, Witt, you can give me your email address and I can send you nanoscope 7.3 manual. Since deflection sensitivity is calculated as delta_z/delta_deflection, so you just need to know deflection error (delta_deflection) and no need to know absolution deflection value. We also have a manual on PFM, but D33 measurement is not straightforwardly written
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Hi, Steve, One recently posted question asked where to find peak force setpoint in offline analysis, somehow the post was archived to off-topic, I tried in nanoscope analysis but also can't find imaging setpoint (only engage setpoint is listed), could you please help to answer this? Thanks! The original post is here: http://nanoscaleworld.bruker
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I didn't include any because the publication or protocol I have seen or used were not for tip coating (we used for glass or PDMS and thus no need covalent bonding) or blocking purpose (we used two-end functional PEG as crosslinker to do single molecule force spectroscopy)... if you are still interested in, I can provide you some references. I guess