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Hi, Ashkan, It seems my post also didn't go through and have to try again :( if your cells measured 3-4um high in air, they could be higher in liquid and that's pretty challenging for normal setup. Also tapping mode doesn't help to reduce lateral force in such case since the side wall of the tip or even cantilever will actually hit the cells
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Hi, Ashkan, if your cells measured 3-4um high in air, they could be higher in liquid and that's pretty challenging for normal setup. Also tapping mode doesn't help to reduce lateral force in such case since the side wall of the tip or even cantilever will actually hit the cells and move them away. ScanAsyst fluid tips are not optimized for fluid
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Hi, Ashkan, I guess you mean you wanna image the cells in tapping mode but would measure mechanical properties under force mode (force curve or force volume or QNM if you have the option). Could you first specify what cell type are you working on? If you are working on adherent type cells, growing them on glass (pretreat the glass with collagen or fibronectin
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Hi, Hosop, Though your post is some while ago, hope you have already solved your porblem, just some quick reply to your questions. In absolute method, the characterizer sample is refered to as RS sample in this post: http://nanoscaleworld.bruker-axs.com/nanoscaleworld/forums/t/792.aspx , you need to use the captured data on this sample as the reference
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Hi, Dalia, you can find the app note here: http://nanoscaleworld.bruker-axs.com/nanoscaleworld/media/p/150.aspx Enjoy reading! LA
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Hi, Ashkan, It depends on how locally or globally you wanna measure the modulus. Usually a soft cantilever is commonly used but the tip shape can vary a lot (it also depends on what contact mechanics model you wanna apply to analyze and extract modulus from your data). Generally speaking, to measure the whole cell property, a sphere bead (diameter range
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Hi, Benoit, Sorry for the wrong message I passed on this issue, I have checked in the latest analysis software and it seems indeed the same as what you have encountered. I guess it's a bug in the software that you update in channel 2 but the result appears in channel 1. You may wanna try other force volume analysis software or just simply change
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Hi, Benoit, In that case, since E is proportional to 1/sqrt(r) according to DMT model, it should be easy to update your E in filters->image math. Set Scaling Factor A to sqrt(r1/r2) and excute (r1 is the default tip radius and r2 is the calibrated tip radius). you can access to all the parameters in file->display properties of..., both deflection
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Btw, E is especially nonliear to d, but to k and r, it can somehow be linearly corrected. And all there discussion are based on your have wrongly calibrated k, r, d as input in your file. If you didn't have calibrated d and k (r will be some default number I guess), the unit of modulus channel will be in Arb (arbitrary unit I guess), then I am not
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Hi, Benoit, To switch from nm to nN in force volume, you should be able to do this in Commands -> set sensitivity, just update the sensitivity from nm/V to nN/V by multiplying the calibrated spring constant with the original deflection sensitivity value. Regarding the software for analyzing force curves in force volume, there is an old post you can