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Hi Luis, If you're interested in knowing more about stress relaxation curve acquisition, you may contact my colleague who picked up the AFM work a couple years ago; Hesam Babahosseini at heb10001@vt.edu. I'm sure he'd be willing to help you with any questions regarding this topic. Also, please take a chance to glance through some of our
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Attached is the graph that i was referring to. Thanks!
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I can't seem to upload/copy my figure that shows the drifting that i'm experiencing. Is there a way to make this possible?
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Hello, I've been running into an issue recently while performing my usual stress relaxation response tests on di3100 afm on live cells. I set my force script to move my z-scanner about 1.5 um into my sample, forcing indentation and causing about 150nm deflection in my cantilever, and then hold the z-scanner position constant for 60s before i retract
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Hello, I'm working with both a di3100 and multimode V afms. Recently, i've been getting the following "internal error" message from both systems: (via multimode afm) FATAL ERROR: SEtranslator Fault D:\V613r1\Z.exe 02/09/2005 14:08:40 NanoScope (R) IV 6.13R1 Quadrexed MultiMode (7039ev.scn), D:\CAPTURE\Alperen.wks Run 12/12/2010 15
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Stefan, I am currently using Olympus TR400PSA triangular cantilevers.( http://www.asylumresearch.com/Probe/TR400PSA,Olympus )There are two tips on each side of the cantilever( k=0.02 and k=0.06). I notice that my 0.06 N/m cantilever seems to give a fine sum all the time.However,recently for all my 0.02 tips(which i've been using in my cell research
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Hello, I was wondering if anyone has a solution or knows the reason for the following problem: I am mounting a triangular shape AFM cantilever(k=0.02 N/m; soft) onto my fluid probe holder and aligning the laser towards the free end tip in air.What i usually get as a sum is around 3.0-4.0V. Soon thereafter, I place a drop of fluid on my cantilever tip
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I think i found a solution to the issue: Due to the effects of fluid, it is necessary to decrease the "minimize engage gain" value found in the scan parameters list window. On average, the value is set at about 3.It is advised to minimize this value to approximately 0.4.One should also try to decrease the integral and proportional gains(0
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Hello, I'm running some force curve tests in liquid medium(via fluid meniscus) with the di3100 afm. My problem is that I can't seem to engage properly on my sample.After aligning my laser onto my cantilever tip (sum~2.5-3.5) and focusing on my surface(in fluid), I hit the engage button. What i notice is that most of the time after doing the
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Hello Stefan, Thank you once again for the reference on the strip chart procedure.I do get convincing results.I would like to extend a question to you: Do you or anyone you may know have experience in performing force curves in liquid medium with the dimension3100 afm? I'm having some issues with the overall setup.Specifically, i'm trying to