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Perfect! Thank you very much. Seyed
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Thanks for all your helps. It worked and now I can create AFM topography and optical images as a two channel AFM format. My purpose of doing this was to have the optical image with the AFM so that I could draw a section line in one image which could be synchronized on the other. However, it seems that the Nanoscope software is not capable of doing it
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I've tried both options you mentioned for import but the result is still the same. Is there any other trick that may seem obvious to you I am missing? Seyed
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The export function (top blue icon) exports only AFM channel but not the optical. I created the ROI and then "export image" --> "Single AFM File (First 8 Channels)" --> then clicked on "Export Layer Images". The saved file has only the AFM image but not the optical. Please let me know if I am not doing it right. Seyed
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Your explanations helped me in terms of understanding how the color scale works and that it’s not as simple as I thought. However, it’s too much work for what I am going to do. I have to think of something else then. What I want to do is to get a line profile of AFM topography and plot it with the line profile of an optical image I overlaid
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Can someone let me know how to find the conversion factor from the color scale (pixel intensity) to height? I capture an image of AFM topography and use it in different software. I have to convert the pixel intensity back to height again. I have a BioScope Catalyst machine and Nanoscope 8.0 software. Thanks Seyed Tadayyon