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We're using a Dimension ICON AFM. I have two questions really. The first, is the DarkLift feature an add-on we need to purchase? Secondly, is there a way to use the SAM V breakout box to switch the laser power on and off? We would like to perform laser light switching tests in C-AFM with the cantilever touching a spot for a few seconds. Ideally
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You can try to treat the mica surface first. Apply a low concentration of poly-L-lysine, then rinse off well with water. This will make it positive. Or, try immersing in MgCl for a couple hours to exchange the K+ ions with Mg2+ ions. Then rinse again. This usually makes the surface positive.
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Hi Marjan- Use a piece of tape to peel off the top and reveal a fresh layer. Apply your aqueous solution to the mica. Potassium ions on the mica surface will easily be displaced by your solution, leaving a negative surface charge on the mica which binds most strongly to biopolymers with cationic side groups (DNA, for example, needs Mg2+ or poly lysine
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Hello- I understand if this is prying too much, but it is regarding the long obsolete NSIIIa controller so I thought I'd ask. We would like to take control over the X/Y by feeding in our own signal just before the HV amplification on the X/Y board. There is an analog switch (DG419) situated just before a buffer that leads into the HV amplifiers
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Hello- We are doing some custom KPFM measurements using an old D3000 AFM with a NS3a controller. The system is operating in Contact mode (feedback and driving handled externally), and we input the surface potential through Aux D. But we would like to have two Aux inputs (say Aux D and Aux B). I thought there were more than 2 ADCs in the controller,
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Hello- Is is possible to attenuate the min/max range of the ADCs linked to the front panel BNCs of the Nanoscope V controller? Thanks. -Ray
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Stefan, that sounds like an ideal solution, thanks. This way I can disconnect my external signal, or simply ground it, and still use the Nanoscope controller regularly. I'll give that a shot. -Ray
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Thanks for the quick response Stephen- After thinking about it I realized the first idea can't work. BTW, I meant to unseat the OP27 op-amp that produces the LV Z not the HV op-amp. I believe this would leave all other relevant connections intact, and I would simply insert my LV signal in the holder at the output pin to emulate the LV op-amp. I'll
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Hello- I'm sure you get this all the time. I, like many an AFMer worldwide, would LOVE to use an external DAC/ADC to acquire the deflection signal and drive the Z piezo up and down on a Nanoscope IIIa. Getting the deflection is trivial, but inserting a LV Z, now that's a-whole-nother issue many have struggled with. So, after perusing the Z board
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For example, NanoScope Analysis v1.40r1, will it work on files acquired with v4 and v5 Nanoscope Software? Thank you.