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Hi Ang, Do you mean plane fit? I would plane fit it XY mode, 1st order fitting, no thresholding and then use the low pass filter on it. The scan size was increased to 2.5 um as I read in one of the Bruker manuals that a larger scan size would make it more accurate for tip qualification and so would the low pass filtering. Regards, Warren.
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Hi Bede, We are using ScanAsyst Fluid probes. We do get this when we install a fresh new probe; it sounds like the Ti roughness sample must be contaminated. From your advice, it sounds like we should try to image the calibration grid with a fresh probe? I will try to image the calibration grid as soon as I get a chance as the AFM is being moved this
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Hi Bede, Thanks for your reply. I don't image the Ti roughness sample in liquid, it is imaged in air. I wasn't so clear in my post. The reason I use PFQNM in liquid mode is because right after I estimate the tip diameter using the Ti roughness sample, I switch over to imaging live cells in glass petri dishes. Here is a link of a typical roughness
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Hi, I've been getting big scan lines across my Ti roughness characteriser sample, when I image it consistently and would like to know what could be the cause of this? I've been using the PFQNM mode in fluid and am using the Ti sample for estimating the tip diameter. To do this, I would image the Ti sample in PFQNM (fluid mode) in air, then switch
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Hi, I am using the Bruker Catalyst AFM. I'm trying to measure cell elasticity using the indentation method using point and shoot. How do I analyse the curves I get from each indentation point using Nanoscope Analysis v 1.40? I want to obtain the exact numbers; i.e. I want to get the deformation/reduced Youngs modulus values at those indentation