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Hi, i imaged a planar glass surface in liquid and a clean image was obtained. So there's a big problem with imaging the fibronectin protein for whatever reason. I'm having no problems imaging this protein on a 21 nm silica rough surface, but when it comes to a planar surface, it doesn't seem to work.
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Hi, I will try imaging the glass slide by itself in liquid mode tomorrow and let you know how it goes. Thanks
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Hi, I've tried imaging one of my samples where I had a glass substrate immersed in a 30 ug/ml fibronectin solution. The glass was just a planar surface. No modifications were made to it. I'm just looking at the protein adsorption on its surface. I already imaged this protein on 21 nm silica nanorough surfaces in liquid mode and the images came