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Thanks Teddy for your answer. I asked for the smallest spot size (in um) of the laser on the cantilever, not the focussing aperture.
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It says: " Enter the URL where an existing file resides." So I just copied the physical address in my computer. Hope it works.
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I would also like to highlight that the PFQNM experiments I did on Bioscope catalyst was 2 yrs ago, when I was in India. Since then I have not used any system with PFQNM. Probably, it was a bug that has already been fixed. By the way, your website is definitely not user-friendly. I can't even upload a simple .jpg file from my computer. Strangely
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But even without any flattening, negative value is observed in Stiffness and Log DMT modulus channels. Another issue is that, it barely showed any difference between proteins and Mica substrate.
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How do I upload a nanoscope file here?
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Thanks Bede for your reply. How am I supposed to work with the modulus channel (or any channel) without flattening it?
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PFQNM ramping speeds are about 2000 times faster than Point & Shoot ramping speed. How can you even think of comparing them? If you to compare force curves with PFQNM data, you need the PeakForce Capture option.
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Even I have seen crappy results with PFQNM on Bioscope Catalyst. I don't understand why one should manipulate the value of deflection sensitivity (DS) values just to get good results. Once, the DS and spring constant has been calibrated, the only parameter that can be tweaked is the modulus limit and tip radius. The problem mainly appears because
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Is it a RF diode laser? How about its interference noise?