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Ashok, You can predict the indentation under different conditions using the common DMT/Hertz indentation model: F = (4/3)*E/(1-v^2)*(R^0.5)*(d^1.5) where: F=Force, E=Elastic modulus, v= Poisson's ratio, R=Tip radius, d=indentation depth (i.e. deformation). It is often assumed that v=0.5, though in fact it's not usually well known. An important
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The Biolevers are, in my experience, very difficult to use because they are so thin they they like to curl over on themselves when put in liquid. This can be hard to distinguish from the cantilever breaking because the cantilever can seem to disappear. Getting them to uncurl can be impossible. The only workaround I can suggest is to be very careful
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Hi Danielle, An elastic modulus of 1kPa really is very soft. All the papers that I've read measuring moduli in that range report using cantilevers that have been modified with microspheres in the range of several micrometers in diameter. If you start doing some rough calculations you start to see why. A common indentation model is the DMT/Hertz
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If you haven't downloaded the App Note linked above then I suggest you start there. It gives a good starting point to selecting a method, considering both technical and practical issues. It includes a brief description of the uncertainty of the methods too. The RSI publication linked at the bottom of my post provides some idea of the best case uncertainty
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Please also refer to our brochure for the Perfusing Stage Incubator, which I just posted under the Brochures section here . The brochure gives more information about the capabilities and specifications of the accessory and also gives more applications examples. -Ben
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Brochure describing Bruker's BioScope Catalyst Perfusing Stage Incubator accessory.
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Spring constant calibration is a very important topic for anyone desiring to measure quantitative material properties or any other kind of quantitative force measurements using AFM. It is also often a confusing topic for those just getting started. So I just want to gather together here what I believe to be good starting resources for those who want
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Abstract Uncertainty in cantilever spring constants is a critical issue in atomic force microscopy (AFM) force measurements. Though numerous methods exist for calibrating cantilever spring constants, the accuracy of these methods can be limited by both the physical models themselves as well as uncertainties in their experimental implementation. Here
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Veeco recently released a new Perfusing Stage Incubator accessory for the BioScope Catalyst. It enables long term maintenance of cells in situ on the BioScope Catalyst. Please see the article just published in Microscopy and Analysis for more details ( Link here ). Abstract Atomic force microscopy has proven itself to be a valuable technique for cell