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I am using PF-QNM (on a Bioscope Catalyst) to measure the elasticity of cancer cells. I have tried to use the Peak Force Capture to extract the force curves for offline analysis, but the curves look like the image below. The extend curve in this file seems to have both the approach and withdrawal from the surface. I have previously had success extracting force curves using HSDC, but wanted to try to extract the force curves for the whole scan.
I have had trouble processing these curves in the Nanoscope analysis - it fits the very tail end of the blue curve (on the right). Is there an easy way to get these curves into a format that can be batch processed? Or is there some reason the force curve looks like this?
Thanks,
Mike
Dear Mike,
I had a very similar problem (http://nanoscaleworld.bruker-axs.com/nanoscaleworld/forums/t/1498.aspx). At the beginning I had the feeling that automatized gains improved the outcome, but later I found that there are situations where it doesn't help you out. Seems to be an unsolved problem.
Dietmar
Hi Mike,
There is a new feature in Nanoscope V1.5 called "Sync Distance New".
This is accessed using the "Show all" option on the scanning parameters, and allows the peakforce triggering position to be set manually. This should help if you have any issues with your force curves looking like this in realtime, when an "AutoConfig" operation does not help. I presume this would also fix the curves captured by PFC.
This might also help with your issue too Dietmar.
Cheers,
Ash
Dear Ash,
Thanks a lot. Sounds promising. However, it happened to me that the live force curves looked fine, but the HSDC- of PFC-curves didn't. I think this might not help to overcome this problem?!
Thanks for the replies!
I was using Nanoscope v1.4 previously, and am installing v1.5 right now. I will check to see if this new feature solves my issues.
It seems like v1.5 was all I needed - it handles to Peak Force Capture files very well. Quite an upgrade!
Thanks for your help!
May I ask, where does one obtain v1.5? I have exactly the problem you described.
You can download it from here: ftp://sboftp.bruker-nano.com/outgoing/GPTech/Software/NanoScope_Analysis/
It took a while for me to find - there was a link in the comments on the page with instructions to download v1.4.
Hope this helps!