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Why is it difficulty to image live cell with PeakForce QNM if it can control the image force at a very low level?
Because operating at a low force (so far I could go down to 26 pN) is not enough to properly image such sample. You also need a high drive amplitude (if possible 1um) to pull off during each oscillation. Also the drive frequency should not be too high (if possible less than 1 kHz). And getting a proper tracking in topography doesn't not mean that you will accurately measure the meachanical properties. For instance to get the proper Young's modulus, it is recommended to apply a loading force of ~200-300 pN with a 0.1 N/m probe.
Alex.