Forums
Bruker Media
Community Media
Bruker AFM Probes
SPM Digest
Application Notes
NanoTheater
Website
中文
Brochures & Datasheets
Publications
Probes Catalog
Events
Manuals & Documentation
Presentations
Guide to AFM Modes
News
Journal Club
Webinars & Video
Nanovations
Other
In agreement with the customer, I put this discussion on the forum. Thanks in advance for any advice that could help Yun:
Hello Alex and Patrick,Thanks for your answers. It's glad to hear that we set the scripts correctly. However, I'm still doubtful about the plots under setpoint ramp mode. Is there any signal channel can be used to record the actual deflection, instead of the deflection error? If there is, then I could really see the deflection increases linearly at the pre-set ramp velocity.And I'd also like consult you about our experimental setup. Please correct me if I'm wrong.
· Firstly, since I'm approaching the bacterial probe to the inert surface, the "fetch molecule" step can be skipped. Am I right at this point?
· And script 1 and 2 were supposed to do the same job: keep the cell under a constant force for some time. Hence, I would expect to see the similar plots from Height Sensor channel during the surface delay for both scripts.
· Do I have any other option to achieve the same goal with the afm?
Sorry for bothering you again with a bunch of new questions. Looking forward to your reply. Thanks in advance.Best wishes,Yun
Hi Alex,Thanks man. You already helped me a lot. Wish you a nice weekend!Best wishes,Yun
Click to show quoted textOn 21-7-2011 19:08, Alex wrote: Sorry for the delay, guys. I haven't used this script utility for a while (last time it was 4 years ago on the Bioscope 2!) but I can briefly answer the 2 questions: 1) Yes, according to the parameters and the general shape of the curve, I think script 2 was set up properly. 2) Unfortunately there is no way to save individually all the scripts, just the entire experiments. Regards, Alex. -----Original Message----- From: Patrick Markus Sent: Monday, July 18, 2011 3:38 PM To: Alexandre Berquand Subject: FW: Questions about the Scripting Mode of AFM Hi Alex, Do you happen to have some time to answer the questions from below? Samuel is out-of-office till end of this week. Patrick Markus Sales and Applications Engineer, Benelux Nano Surfaces Division Bruker Nederland BV Bruynvisweg 18 Patrick.Markus@bruker-nano.com 1531 AZ Wormer Phone: +31 (0)6 5175 9884 The Netherlands Fax: +31 (0)75 628 9771 www.bruker-axs.com -----Original Message----- From: Patrick Markus Sent: Monday, July 18, 2011 3:36 PM To: Samuel Lesko Subject: FW: Questions about the Scripting Mode of AFM Hi Samuel, Are you able to help this BioScope catalyst user with his questions? Maybe he did already send you this e-mail as my out-of-office is on. Regards, Patrick Markus Sales and Applications Engineer, Benelux Nano Surfaces Division Bruker Nederland BV Bruynvisweg 18 Patrick.Markus@bruker-nano.com 1531 AZ Wormer Phone: +31 (0)6 5175 9884 The Netherlands Fax: +31 (0)75 628 9771 www.bruker-axs.com -----Original Message----- From: Yun Chen [mailto:yun.chen@med.umcg.nl] Sent: Monday, July 18, 2011 1:56 PM To: Patrick Markus Subject: Questions about the Scripting Mode of AFM Hello Patrick, This is Yun from BME, UMCG. Again we encountered some difficulties with our newest AFM (Bioscope Catalyst with ScanAsyst). And Joop suggested that I should directly turn to you for suggestions. This time it's about the scripting mode, and I put down our questions in details within the attached doc. Hopefully you could help us out again like you always did. And we also failed to locate the sections about the scripting mode in the manuals for this AFM, while it was mentioned in the manual for the older machine. Since the interfaces of the scripting mode in NanoScope 7 and 8.1 are slightly different, we are not completely sure if it still functions in the same way or not. Looking forward to your reply. Thanks in advance. Best wishes, Yun DISCLAIMER: This email and any attachments are confidential, may be attorney-client privileged and are intended only for the use of the addressee. Unauthorized use, distribution or copying is forbidden and may be unlawful. If you have received this email in error, please notify the sender immediately by return email and delete all copies of this message and any attachments from your computer. Thank you. scan channel *The Z closed loop was always turned on in measurements. Plots recorded by script 1 (NP-10 Cantilever at Glass Slide in PBS 10mM buffer) Script 2: Setpoint channel Plots recorded by script 1 (NP-10 Cantilever at Glass Slide in PBS 10mM buffer) . Questions: Since we used NP-10 cantilever against glass slide as a control for the later bacterial probe, we did not expect to see any significant piezotube position change during the surface delay. For this reason, the plots from script 1 seem to be reasonable; however, the plots from script 2 are quite different. So we are not sure if script 2 was properly set up or not. Also we are wondering if it is possible to save scripts as individual files for later use. Currently we can only do it by saving the whole experiment. | Post Points: 10
Sorry for the delay, guys. I haven't used this script utility for a while (last time it was 4 years ago on the Bioscope 2!) but I can briefly answer the 2 questions:
1) Yes, according to the parameters and the general shape of the curve, I think script 2 was set up properly.
2) Unfortunately there is no way to save individually all the scripts, just the entire experiments.
Regards,
Alex.
-----Original Message-----
From: Patrick Markus
Sent: Monday, July 18, 2011 3:38 PM
To: Alexandre Berquand
Subject: FW: Questions about the Scripting Mode of AFM
Hi Alex,
Do you happen to have some time to answer the questions from below?
Samuel is out-of-office till end of this week.
Patrick Markus
Sales and Applications Engineer, Benelux
Nano Surfaces Division
Bruker Nederland BV
Bruynvisweg 18 Patrick.Markus@bruker-nano.com
1531 AZ Wormer Phone: +31 (0)6 5175 9884
The Netherlands Fax: +31 (0)75 628 9771
www.bruker-axs.com
Sent: Monday, July 18, 2011 3:36 PM
To: Samuel Lesko
Hi Samuel,
Are you able to help this BioScope catalyst user with his questions?
Maybe he did already send you this e-mail as my out-of-office is on.
Sales and Applications Engineer, Benelux Nano Surfaces Division Bruker Nederland BV
From: Yun Chen [mailto:yun.chen@med.umcg.nl]
Sent: Monday, July 18, 2011 1:56 PM
To: Patrick Markus
Subject: Questions about the Scripting Mode of AFM
Hello Patrick,
This is Yun from BME, UMCG. Again we encountered some difficulties with our newest AFM (Bioscope Catalyst with ScanAsyst). And Joop suggested that I should directly turn to you for suggestions. This time it's about the scripting mode, and I put down our questions in details within the attached doc. Hopefully you could help us out again like you always did.
And we also failed to locate the sections about the scripting mode in the manuals for this AFM, while it was mentioned in the manual for the older machine. Since the interfaces of the scripting mode in NanoScope 7 and 8.1 are slightly different, we are not completely sure if it still functions in the same way or not.
Looking forward to your reply. Thanks in advance.
Best wishes,
Yun
DISCLAIMER:
This email and any attachments are confidential, may be attorney-client privileged and are intended only for the use of the addressee. Unauthorized use, distribution or copying is forbidden and may be unlawful. If you have received this email in error, please notify the sender immediately by return email and delete all copies of this message and any attachments from your computer. Thank you.
scan channel
*The Z closed loop was always turned on in measurements.
Plots recorded by script 1 (NP-10 Cantilever at Glass Slide in PBS 10mM buffer)
Script 2: Setpoint channel
.
Questions: