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Hi, All
I was a user of nanowizard II before and always got stiffness and adhesion channels together with topography out of force volume mapping. Recently I switch to use a MultiMode system but don't understand what force volume result means. Especially the unit of force volume channel is nm but shouldn't nN make more sense? Anyone can show me how to seperately get stiffness and adhesion maps out of force volume in nanoscope system? Any help is appreciated.
Albert
Hi, Albert,
FV channel in nanoscope software does not directly seperate into different property channels as Nanowizard II does. Instead, it shows the raw deflection data (thus in nm unit) at particular fixed z positions for each pixel. To extract stiffness and adhesion maps out from FV channel, you will need to choose proper z positions within indentation or adhesion regions manually (for details you can refer to manual or help file of nanoscope software). A more reliable and robust way to quantify different properties if you have to stick to FV method is to export the force data and analyze individual force curves using your own algorism or a trustable third party software. If you have access to use peak force QNM, it will be much more easier and faster and higher resolution for your application than FV.
LA
I strongly recommend Open Fovea, which I advertised in the following paper:
Atomic Force Microscopy Imaging of Living Cells Alexandre Berquand,
Atomic Force Microscopy Imaging of Living Cells
Alexandre Berquand,
Charles Roduit, Sandor Kasas, Andreas Holloschi, Leslie Ponce and Mathias Hafner
In Microscopy Today (ref. MTO18_6-00095)
Looking at figure 4 will give you a good idea how you can display the data points: grey scale for topography, color scale for Young's modulus and red arrows for specific unbinding events.
Alex.