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Hi,
I'm using AFM to measure the Young's modulus (E) of cells. I typically use the Hertz or Sneddon's equation (which require a tip angle) to calculate E based on force-displacement data. For most tip geometries (sphere, square pyramid, cone) the calculation is pretty straight forward. However, for the MPP-32100-10 tip the geometry is described as Rotated (Symmetric) and there are 3 different angles listed (front, back and side angle).
What equation/ angle would you recommend using for data analysis? Thanks!
I think my answer didn't go through...
Once again: I hardly use Hertz on cells but in that case, the equation is fairly easy. If using Sneddon, the correct anglt should be the BACK angle (25 deg. in that case).
May I ask your opinion about those probes (I never had a chance to test them) in terms of measured modulus and quality/stability of imaging?
Thanks and good luck!
Alex.
Thanks for your response Alex.
I haven't use these tips for imaging, just for a few measurements of modulus.
I found them to give values similar to other pyramidal tips but they seem to be more damaging to the cells.
Usually the tips which are really damaging to the cells are the ones:
1) that have a silicone (not Si3N4 but pure silicone) ending.
2) that have a pyramidal shape (like the OTR for instance, which are very good to image proteins but not suitable for cells).
Let me know how it goes.
Good luck,