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we're having a weird phenomenon. We calculate the spring constant of the tipusing the thermal fluctuation method. It generally works well but lately wehave a strange result. Tips with K > 4 n/m we used before appear to have thermal fluctuation signal which is much reduced. The peak of the FFT signal drops almost an orderof magnitude with the calculated spring constant going up 2-5 timeswhat it used to be. This happen even at 10Mhz sampling rate, on more thanone machine and with tips of 2 different manufacturers! The strength of the laser signal from the tips themselves remain the same.When we measure with the "stiffer" tips they appear to be the same as before.Do you know of any process that can cause the thermal fluctuation signal toappear/or actually to be reduced?