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Hi,
We have a new Dimension Icon in our lab. It works well for imaging in air, but recently, we got some issues when we tried imaging in fluid. We used the fluid cantilever holder and followed each step to setup the experiment. The SUM signal is about 3 to 4, and the the autocompensation for focus in fluid is also selected. However, when I tried to engage on the surface after I navigated the tip about 1mm above the sample suface, the software gave an error message after pre-egage test, and z approaching failed. The error messages said "Crash protection has detected a X (sometimes Y) sensor below min crash." and "Z stage downwardmoving failed. The scanner may hit the surface because of wrong surface focus." At first we thought the scanner might be too close to the surface, but we got the same message even if we lifted the scanner apparently high from the surface. We tried different samples and tips (hard and soft ones), all came out with the same message. Does anyone have similar problem before?
Thanks a lot for your help!
Hi Coldfire,
If you have not done so already please contact Bruker support for this issue at the following email or phone number:
AFMsupport@Bruker-Nano.com or 1-805-967-1400
Through the above we can have someone troubleshoot the problem and get you up and running in fluid.
We faced similar problem in our setup. When we contacted technical support, we were informed that we should be using a micro-pipette to put a drop of liquid on the cantilever after bringing it approx 1-2mm near the sample. That drop will join the liquid sample and form a continuous capillary of the liquid from the cantilever to the sample. Now you can focus once again and then engage.