The Nanoscale World

QNM on living diatoms

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Posted: Sun, Dec 18 2011 2:12 AM

I posted the topic a few months ago. Just to inform you that the publication is eventually available in Journal of Phycology. Here is the abstract:

QUANTITATIVE NANOMECHANICAL MAPPING OF MARINE DIATOM IN SEAWATER

USING PEAK FORCE TAPPING ATOMIC FORCE MICROSCOPY

Galja Pletikapic, Alexandre Berquand, Tea Misic Radic and Vesna Svetlicic

It is generally accepted that a diatom cell wall is

characterized by a siliceous skeleton covered by an

organic envelope essentially composed of polysaccharides

and proteins. Understanding of how the organic

component is associated with the silica structure provides

an important insight into the biomineralization

process and patterning on the cellular level. Using a

novel atomic force microscopy (AFM) imaging technique

(Peak Force Tapping), we characterized nanomechanical

properties (elasticity and deformation) of

a weakly silicified marine diatom

 

Cylindrotheca closterium

(Ehrenb.) Reimann et J. C. Lewin (strain CCNA1).

The nanomechanical properties were measured over

the entire cell surface in seawater at a resolution that

was not achieved previously. The fibulae were the

stiffest (200 MPa) and the least deformable (only

1 nm). Girdle band region appeared as a series of

parallel stripes characterized by two sets of values of

Young’s modulus and deformation: one for silica

stripes (43.7 Mpa, 3.7 nm) and the other between the

stripes (21.3 MPa, 13.4 nm). The valve region was

complex with average values of Young’s modulus

(29.8 MPa) and deformation (10.2 nm) with high

standard deviations. After acid treatment, we identified

15 nm sized silica spheres in the valve region

connecting raphe with the girdle bands. The silica

spheres were neither fused together nor forming a

nanopattern. A cell wall model is proposed with individual

silica nanoparticles incorporated in an organic

matrix. Such organization of girdle band and valve

regions enables the high flexibility needed for movement

and adaptation to different environments while

maintaining the integrity of the cell.

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