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Hi Moritz, In cases such as the one that you describe, it is difficult to determine the true deformation. The simple algorithm that we use to calculate the deformation may not match the true deformation, but it has the advantages that it is easy to describe and calculate in real-time. As the application...
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That is generally due to poor deflection sensitivity calibration. If the deflection sensitivity is too large, the change in cantilever bending can be erroneously calculated to be larger than the Z piezo motion. This causes the deformation calculation to return negative numbers and will cause problems...
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The specific situation that caused us to choose 85% is the sort of long range repulsion or baseline error shown in this curve: This kind of thing is fairly common in fluid (double layer forces). It is a little strange to call it noise, but it is not part of the sample deformation either. If your curves...