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When you collected your data, the Offline Planefit parameter (called "OL Planefit" in the software) was set to "Offset." This will offset the average Z value of the image to the center of the data scale in the saved image. So, it centered the modulus values of the image at 0 MPa when the image was saved. This is why your modulus
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Hi Hari, There are 2 primary differences between the NanoScope III and IIIa controllers - The IIIa controller allows the use of "Z Modulation," which was used for TappingMode in fluid on the AFM systems at that time that used the Dimension scanner, such as the Dimension 3000, 3100, 5000, and original BioScope. The fluid cantilever holder did
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Hi Pete The Dimension ICON can adjust the drive frequency of the piezo in the cantilever holder to keep the cantilever oscillating at resonance during the Lift scan. It does this by using a feedback loop to keep the cantilever phase lag at 90 degrees during the Interleave scan. So, instead of letting the cantilever resonance frequency shift due to magnetic
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Hello Hikari81 Q1 - In (a), you are looking a the blue line to measure 10V, which is the trace direction (left to right scan). However, you are collecting the data in the retrace direction (right to left) for the saved image. The frictional contrast will be due to the lateral bending of the cantilever, which will be in opposite directions for the trace
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Hi Harman, Thanks for your question and for posting the photos. One concern that I have from looking at the photos is that it looks like the Z range of your scanner is only about 2um, although the software says 5um. This could be causing your problem. If you look at the "Z sensor" vs "Z" curve in normalcurve.jpg, you can see that
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Hi Mac, If you are using the factory setting for ScanAsyst and PeakForce QNM, they may have different engage settings. Go to the Engage Settings under the Microscope pull down menu, and check the Engage Mode parameter under the Smart Engage list. There are two possible settings for this parameter - Smart and Standard. ScanAsyst is set to Smart in the
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Hi Sandeep, If you have magnetic probes with a 0.2N/m spring constant, you may still be able to conduct MFM. There are different ways to approach this. MFM is usually conducted by LiftMode which will collect a line of topography data, and then use that information on the following line to maintain a constant tip-sample separation when detecting the
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Hi Djuenne, Sure. You can send the images and details to me at john.thornton@bruker-nano.com . You should be able to get good cell images with the ICON as well. Best wishes, John
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Hi Saumil I am glad that you found access to a system that you can use. If it is a closed loop system with version 5 on it, then it probably also has version 6 on it as well, depending on the configuration. Version 6 is where you would find the Nanoman interface that you were trying to use previously. Can you tell me what the system model and controller
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Hi Denis, Try going to the Microscope pull down menu, then choosing Calibrate, then Scanner. "Minimum scan speed" is at the bottom of the list of calibration parameters. I hope this helps. John Thornton