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As you said, the heights of the particles are not changed when imaging a 10-um size image (with a calibrated vertical engage E scanner) when the peak force setpoint is set at ~ 1 nN force and then at ~ 3 nN force. However, the particle height distributions are narrower in the higher peak force setpoint (and on auto mode) compared to the lower fixed

Hi All, I have the following problem with the peak force setpoint changing significantly during a 10-um scan size imaging (i.e changing from ~ 500 pN to about 3-4 nN's), while this problem does not show up when imaging the same sample at smaller scan sizes (i.e. less than 5 um). The sample contains proteins attached to about ~50 nm particles on