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Hi, Nithya This is James from Bruker. Would you be able to send me your contact info. Maybe we can setup a remote session if your AFM computer is connected to an Internet. Please email me at james.shaw@bruker-nano.com Best, James
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Hi, Dejan What type of o-ring are you using? Usually to get a good seal. I engage in air first. Then I inject the fluid through the tubing to make sure there is no leak. I like to use 1 ml syringe for my in-port and the silicon tubing with stop clamp on the out let. To avaid air bubble during buffer exchange when changing the syringe, I wet the in-port
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Biopolymers. 1996 Mar;38(3):355-66. Imaging polysaccharides by atomic force microscopy. Kirby AR , Gunning AP , Morris VJ . Source Institute of Food Research, Norwich Laboratory, UK. Abstract Techniques have been developed for the routine reliable imaging of polysaccharides by atomic force microscopy (AFM). The polysaccharides are deposited from aqueous
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Hi, Josep I don't think there is a way to modify the setting. The range is either 1 to 100 kHz or 5 to 2000 kHz What type of cantilever are you using? Most of the cantilever will fall with in the those two settings. Are you trying to do something different? -James
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Hi, Raphael I think all of them are possible variables for engagement problems for Tapping in fluid. I will summarize my experience 1. Mica substrate lifting: when the mica detach from the metal puck or single layer lifting can cause problem in engagement. Usually the defl go positive and out of range 2. Tuning to the wrong peak. For the cantilever
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Hi, Raphael I think all of them are possible variables for engagement problems for Tapping in fluid. I will summarize my experience 1. Mica substrate lifting: when the mica detach from the metal puck or single layer lifting can cause problem in engagement. Usually the defl go positive and out of range 2. Tuning to the wrong peak. For the cantilever
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Hi, Raphi Do you think you can shoot me an email: james.shaw@bruker-nano.com Let me see if I can help you offline. Best, James
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I am asking on a behalf of a customer. He said that the images published on our AFM calendar used to be accessible on the nanotheatre. Is it still the case after the migration? Best, James
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Hi, Djuenne My experience with live cell imaging using contact mode Probes: Soft & blunt probes. Generally silicon tip (~2 nm tip radius) are much sharper than silicon nitride tip (~ 10 to 20 nm tip radius). While SNL probes can be soft with silicon nitride cantilevers (down to k ~ 0.06 N/, the probe sharpness often still cause cells to destabilize
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Hi, Joseph Depositing collagen on mica and glass through aspiration and then imaging them in air or in fluid after rehydration in buffer is very common and useful AFM sample preparation for imaging fibrous proteins. However, for people who are interesting in the kinetic of fiber growth, this method would not be so useful. Muller group was able to study